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High-Quality Genome Resource of Bacillus subtilis N4, a Biological Control Agent Isolated from Phoebe bournei.

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Bacillus subtilis strain N4 was an endophyte of isolated from the healthy leaves of Phoebe bournei, and possesses an excellent biocontrol effect on P. bournei stem canker caused by Lasiodiplodia… Click to show full abstract

Bacillus subtilis strain N4 was an endophyte of isolated from the healthy leaves of Phoebe bournei, and possesses an excellent biocontrol effect on P. bournei stem canker caused by Lasiodiplodia theobromae. To understand its biocontrol mechanism, we assembled a high-quality genome of N4 using a combination of second and third generation sequencing methods. The genome of N4 contains only one circular DNA chromosome of 4,218,183 bp length with 43.5% GC content, and 11 secondary metabolite biosynthesis gene clusters such as subtilomycin synthesis exist in the genome. Taken together, this high-quality genome assembly and gene annotation resource will provide better insights into its biocontrol potential against stem canker of P. bournei.

Keywords: quality genome; bacillus subtilis; high quality; phoebe bournei

Journal Title: Phytopathology
Year Published: 2022

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