LAUSR

Objectives: To explore the relationship between peripheral blood monocyte (PBM) subsets and LV remodeling in SHR. Methods: 7-week-old male SHR were randomized to receive 0.5% NaCl (LS) and 8% NaCl (HS) for 12 weeks. BP was measured by tail cuff method. Peripheral blood were collected for flow cytometry analysis at baseline and after 12-week salt intervention. At the end of intervention, all rats were anesthetized for echocardiography measurements and then sacrificed. Morphologic staining (collagen deposition, cardiomyocyte hypertrophy and macrophage infiltration) were performed at time of sacrifice. And the relationship between PBM subsets and LV remodeling were analyzed at last. Results: Compared with LS group, the SBP, heart rates (HR), LV mass (LVM) and LVM index (LVMI) of HS group were significantly increased while body mass (BM) was markedly decreased at the end of 12-week intervention (P < 0.05). Flow cytometry analysis showed that the percentage of CD43++ monocyte in HS group were apparently increased while a reduction of CD43+ monocytes were observed compared with LS group (P < 0.05). Echocardiography measurements demonstrated that the LVEDD and LVEDV were significantly increased (P < 0.05). Moreover, morphologic staining found that a severe myocardial fibrosis, cardiomyocyte hypertrophy and macrophage infiltration were observed in the HS group. And a positive correlation between the percentage of CD43++ monocyte and SBP, LVM, LVMI, LVEDD, LVEDV, myocardial fibrosis, cardiomyocyte hypertrophy and mocrophage infiltration were also observed. Conclusion: The present study demonstrates a positive correlation between the CD43++ monocytes and LV remodeling which indicates a important role of CD43++ monocyte in this process and prompts a valuable therapy target for further study.