LAUSR

Objectives: Our previous studies have proved that ginsenoside Rb1 (gRb1) prevent H2O2 induced HUVECs senescence through SIRT1/eNOS/ROS axis. This study is to explore the effect of gRb1 on ox-LDL induced HUVECs senescence and the role of SIRT1/Beclin-1/autophagy. Methods: Cell senescence was evaluated by PAI-1 expression, cell viability and senescence staining. Autophagy was detected by p62 and LC3 II/LC3 I and confocal microscopy. The acetylation of Beclin-1 and corelation between SIRT1 and Beclin-1 were detected by immunoprecipitation and co-immunoprecipitation. Results: Results showed that pretreatment of gRb1 effective prevent HUVECs from ox-LDL induced PAI-1 up-regulation and increasing positive cells of senescence staining. And gRb1 also restored the reduction of SIRT1 and autophagy which were involved in its anti-senescence effect. Acetylation of Beclin-1 was reduced and correlation of SIRT1 and Beclin-1 was increased by gRb1. Conclusion: In conclusion, our study proved the anti-senescence function of gRb1 and the mechanism involves SIRT1/Beclin-1/autophagy axis. As such, gRb1 may have preventive effect on the generation of atherosclerosis.