LAUSR

Objectives: This study is to observe the change of hypothalamic AngII, AT1R, p38MAPK and p65NF&kgr;B before and after renal denervation on spontaneously hypertensive rats, to observe the effect of renal denervation on MAPK and NFKB signaling pathway. Methods: 36 SHR rats were divided into three groups randomly: SHR blank group, RDN group and sham group. Choose the same age WKY rats as the blank control group (WKY group, n = 12). The SHR RDN group and sham group were given RDN or sham operation, then were killed respectively 1 week and 6 week after RDN (D1 group, n = 6; D6 group, n = 6), as well as 1 week and 6 weeks after sham operation (S1 group, n = 6; S6 group, n = 6). Kidney and hypothalamus were collected for examination. The content of plasma S100B, NSE, kidney NE and hypothalamic AngII were determined by ELISA. The protein expression of p38MAPK, p-p38MAPK and p65NFkB in hypothalamus were determined by Westerm-Blot. The mRNA expression of AT1R and P38MAPK in hypothalamus were detected by real-time PCR. Results: In SHR blank group, the level of blood pressure, the content of hypothalamic AngII, kidney NE, plasma NSE, S100B and the expression of AT1R, p38MAPK, p-p38MAPK and p65NFkB increased significantly compared with WKY group (p < 0.05). The blood pressure in D1 group decreased significantly compared with the S1 group (p < 0.05). The blood pressure in D6 group Conclusion: Renal denervation can significantly decrease the level of NE in kidney and effectively lower blood pressure of SHR rats in a short term. By suppressing the p38MAPK and p65 NFkB signal pathway, renal denervation participates the central antihypertensive mechanism.