LAUSR

Objectives: To identify the mechanism of plasma circulating miRNA-296-5p in essential hypertension (EH) patients. Methods: Circulating miRNA-296-5p of plasma samples from 80 EH cases and 80 non-EH cases were analyzed by qRT-PCR, and its biological information was predicted. Then we carried out ELISA to detect the probable target gene of miRNA-296-5p. Results: There was no statistical difference in age, gender, BMI, basic history and basic laboratory tests (P > 0.05); the circulating miRNA-296-5p levels in EH cases were significantly higher than non-EH cases (P < 0.01); ROC curve analysis found that the area under the curve is 0.8 445, and the 95%CI is 0.7 825∼0.9 064 (P < 0.01); bioinformatics prediction found that TGF-&bgr;1 and ACE2 might be the target gene of miRNA-296-5p, and miRNA-296-5p may regulate TGF-&bgr;/SMAD, PI3K-Akt, Insulin/IGF, MAPK, NOTCH signaling pathways, also regulates some related circRNA; ELISA revealed that plasma TGF-&bgr;1 and ACE2 levels were significantly higher in EH cases (P < 0.01), and the Pearson R are 0.3 176 and 0.4 171 (P < 0.01). Conclusion: Circulating miRNA-296-5p can be a novel biomarker of EH, and may participate in the mechanism of EH by regulating TGF-&bgr;1 and ACE2.