LAUSR

Supplemental Digital Content is Available in the Text. Henle fiber layer, which is composed of photoreceptor axons and Müller cell processes, shows an ambivalent reflective pattern that is detected by directional optical coherence tomography. This study aims to perform volumetric Henle fiber layer mapping on the full extent of macular area with vertical and horizontal directional optical coherence tomography acquisition. Purpose: To perform a macular volumetric and topographic analysis of Henle fiber layer (HFL) from retinal scans acquired by directional optical coherence tomography. Methods: Thirty healthy eyes of 17 subjects were imaged using the Heidelberg spectral-domain optical coherence tomography (Spectralis, Heidelberg Engineering, Heidelberg, Germany) with varied horizontal and vertical pupil entry. Manual segmentation of HFL was performed from retinal sections of horizontally and vertically tilted optical coherence tomography images acquired within macular 20 × 20° area. Total HFL volume, mean HFL thickness, and HFL coverage area within Early Treatment for Diabetic Retinopathy Study grid were calculated from mapped images. Results: Henle fiber layer of 30 eyes were imaged, segmented and mapped. The mean total HFL volume was 0.74 ± 0.08 mm3 with 0.16 ± 0.02 mm3, 0.18 ± 0.03 mm3, 0.17 ± 0.02 mm3, and 0.19 ± 0.03 mm3 for superior, temporal, inferior, and nasal quadrants, respectively. The mean HFL thickness was 26.5 ± 2.9 µm. Central 1-mm macular zone had the highest mean HFL thickness with 51.0 ± 7.6 µm. The HFL coverage that have thickness equal or above to the mean value had a mean 10.771 ± 0.574 mm2 of surface area. Conclusion: Henle fiber layer mapping is a promising tool for structural analysis of HFL. Identifying a normative data of HFL morphology will allow further studies to investigate HFL involvement in various ocular and systemic disorders.