LAUSR

Gap junction (GJ) is concerned with cell growth, differentiation, immune response, as well as many physiological and pathological processes. Cx43, as an important GJ protein, is associated with a variety of diseases. This study investigated the effect of miR-301a-3p in bacterial meningitis by targeting the Cx43 gene. The negative correlation between Cx43 and miR-301a-3p was because of the abnormal expression of related genes. MiR-301a-3p agomir was transfected into astrocytes for higher expression; CCK8 assay and flow cytometry showed that the high expression of miR-301a-3p would inhibit apoptosis and induces proliferation of astrocytes, whereas miR-301a-3p antagomir would inhibit proliferation and induce apoptosis. Bioinformatics analysis showed that Cx43 was the target gene of miR-301a-3p, and dual-luciferase assay and experiments repeated showed that miR-301a-3p regulated the expression of Cx43 on the 3′-untranslated region seed region. Therefore, miR-301a-3p played a biological role in the development of bacterial meningitis by regulating the expression of the target gene Cx43.