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Palo: spatially aware color palette optimization for single-cell and spatial data

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Summary In the exploratory data analysis of single-cell or spatial genomic data, single cells or spatial spots are often visualized using a two-dimensional plot where cell clusters or spot clusters… Click to show full abstract

Summary In the exploratory data analysis of single-cell or spatial genomic data, single cells or spatial spots are often visualized using a two-dimensional plot where cell clusters or spot clusters are marked with different colors. With tens of clusters, current visualization methods often assigns visually similar colors to spatially neighboring clusters, making it hard to identify the distinction between clusters. To address this issue, we developed Palo that optimizes the color palette assignment for single-cell and spatial data in a spatially-aware manner. Palo identifies pairs of clusters that are spatially neighboring to each other and assigns visually distinct colors to those neighboring pairs. We demonstrate that Palo leads to improved visualization in real single-cell and spatial genomic datasets. Availability Palo R package is freely available at https://github.com/Winnie09/Palo. Contact [email protected]

Keywords: cell; spatially aware; cell spatial; color palette; single cell; spatial data

Journal Title: Bioinformatics
Year Published: 2022

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