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Preparation of Polymerase Chain Reaction Template DNA from Mouse Tail Tissue.

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A simple cell or tissue lysate can provide a sufficient quality and amount of template DNA for polymerase chain reaction (PCR). In this protocol, a small piece from the tip… Click to show full abstract

A simple cell or tissue lysate can provide a sufficient quality and amount of template DNA for polymerase chain reaction (PCR). In this protocol, a small piece from the tip of the tail is removed and processed using hot sodium hydroxide and Tris (HotSHOT).

Keywords: template dna; polymerase chain; chain reaction; tissue

Journal Title: Cold Spring Harbor protocols
Year Published: 2019

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