Photo by nci from unsplash
During extraction of aqueous solutions with solvents such as secondary butyl alcohol (isobutanol) or n-butyl alcohol (n-butanol), a proportion of the water molecules is partitioned into the organic phase. Nucleic… Click to show full abstract
During extraction of aqueous solutions with solvents such as secondary butyl alcohol (isobutanol) or n-butyl alcohol (n-butanol), a proportion of the water molecules is partitioned into the organic phase. Nucleic acids remain in the aqueous phase. By performing several sequential cycles of extraction, the volume of a nucleic acid solution can be reduced significantly. This method of concentration can be used to reduce the volume of dilute solutions to the point where the nucleic acid can be recovered easily by precipitation with ethanol.
Journal Title: Cold Spring Harbor protocols
Year Published: 2017
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!