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This protocol describes the isolation of radial glia-like neural progenitors (RGLs) from embryonic and adult mouse brain. Their propagation as an adherent monolayer is greatly supported by use of the… Click to show full abstract
This protocol describes the isolation of radial glia-like neural progenitors (RGLs) from embryonic and adult mouse brain. Their propagation as an adherent monolayer is greatly supported by use of the AK-cyclo(RGDfC) adhesive peptide-conjugate that allows the application of fully defined culture conditions in which epidermal growth factor (EGF) represents the only growth factor supplement. RGL cells maintained that way can be efficiently differentiated into the three major CNS cell types: neurons, astrocytes, and oligodendrocytes.
Journal Title: Cold Spring Harbor protocols
Year Published: 2017
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