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Direct Cyanine-dCTP Labeling of DNA for Microarrays.

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Similar to direct RNA labeling, direct DNA labeling with Cy-dCTP is the simplest and fastest method for labeling DNA. This is a standard Klenow labeling protocol in which Cy-dCTP is… Click to show full abstract

Similar to direct RNA labeling, direct DNA labeling with Cy-dCTP is the simplest and fastest method for labeling DNA. This is a standard Klenow labeling protocol in which Cy-dCTP is incorporated during the labeling reaction. After stopping the reaction, labeled nucleotide is separated from unreacted Cy-dCTP, and Cy3- and Cy5-labeled materials are combined for hybridization. This protocol is suitable for many applications, including detection of copy-number variation, nucleosome mapping, and other location analysis (e.g., chromatin immunoprecipitation [ChIP]-chip).

Keywords: labeling dna; dctp; cyanine dctp; dctp labeling; direct cyanine

Journal Title: Cold Spring Harbor protocols
Year Published: 2019

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