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The one-step approach to bacterial artificial chromosome (BAC) modification requires that only one homology arm be cloned into the shuttle vector (in the example presented here, we use the "A-box").… Click to show full abstract
The one-step approach to bacterial artificial chromosome (BAC) modification requires that only one homology arm be cloned into the shuttle vector (in the example presented here, we use the "A-box"). The homology arm, which in this case lies upstream of the ATG start codon, is amplified by polymerase chain reaction (PCR) using purified BAC DNA as template. The resulting amplification product is then digested with the appropriate restriction endonuclease to render it suitable for cloning into the shuttle vector.
Keywords:
bac;
one step;
bacterial artificial;
homology arm
Journal Title: Cold Spring Harbor protocols
Year Published: 2020
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Journal Title: Cold Spring Harbor protocols
Year Published: 2020
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!