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This protocol differs from other transformation procedures in that the bacterial culture is grown at 18°C rather than the conventional 37°C. Otherwise, the protocol is unremarkable and follows a fairly… Click to show full abstract
This protocol differs from other transformation procedures in that the bacterial culture is grown at 18°C rather than the conventional 37°C. Otherwise, the protocol is unremarkable and follows a fairly standard course. Under standard laboratory conditions, efficiencies of 1 × 108 to 3 × 108 transformed colonies/µg of plasmid DNA are typical.
Keywords:
preparation transformation;
inoue method;
method preparation;
transformation;
competent escherichia;
transformation competent
Journal Title: Cold Spring Harbor protocols
Year Published: 2020
Link to full text (if available)
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Journal Title: Cold Spring Harbor protocols
Year Published: 2020
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!