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Flow cytometry or fluorescence-activated cell sorting (FACS) can be used to identify hybridomas secreting monoclonal antibodies to internal cellular proteins, but the cells must be permeabilized before the hybridoma supernatants… Click to show full abstract
Flow cytometry or fluorescence-activated cell sorting (FACS) can be used to identify hybridomas secreting monoclonal antibodies to internal cellular proteins, but the cells must be permeabilized before the hybridoma supernatants are applied. In using this technique, useful controls are positive and negative cell lines with primary and secondary antibodies as well as positive and negative cell lines with secondary antibody alone.
Keywords:
hybridoma screening;
flow cytometry;
screening antibody;
cytometry;
antibody capture
Journal Title: Cold Spring Harbor protocols
Year Published: 2022
Link to full text (if available)
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Journal Title: Cold Spring Harbor protocols
Year Published: 2022
Link to full text (if available)
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recommendations!