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If the antigen of interest is a cell-surface protein, immunofluorescence can be used to identify hybridomas secreting monoclonal antibodies to these proteins. They can be stained on microscope chamber slides,… Click to show full abstract
If the antigen of interest is a cell-surface protein, immunofluorescence can be used to identify hybridomas secreting monoclonal antibodies to these proteins. They can be stained on microscope chamber slides, flat-bottomed cell culture plates (96, 48, or 24 well), or imaging plates (96 well). This protocol uses 96-well imaging plates.
Keywords:
cell;
hybridoma screening;
cell surface;
screening antibody;
immunofluorescence
Journal Title: Cold Spring Harbor protocols
Year Published: 2022
Link to full text (if available)
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Journal Title: Cold Spring Harbor protocols
Year Published: 2022
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!