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Hybridoma screening by immunofluorescence stainings of whole cells can be adapted to screen for antibodies to internal proteins by permeabilizing the cells before applying the hybridoma supernatants. In this protocol,… Click to show full abstract
Hybridoma screening by immunofluorescence stainings of whole cells can be adapted to screen for antibodies to internal proteins by permeabilizing the cells before applying the hybridoma supernatants. In this protocol, cells are attached to a solid support (glass slides), which makes them easy to manipulate and transfer between different reagent solutions.
Keywords:
capture detection;
hybridoma screening;
screening antibody;
immunofluorescence;
hybridoma;
antibody capture
Journal Title: Cold Spring Harbor protocols
Year Published: 2022
Link to full text (if available)
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Journal Title: Cold Spring Harbor protocols
Year Published: 2022
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!