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Immunoprecipitation is rarely used for screening hybridoma fusions because the assays are tedious and time-consuming. However, it can be useful when working with complex antigens because the precipitated antigen is… Click to show full abstract
Immunoprecipitation is rarely used for screening hybridoma fusions because the assays are tedious and time-consuming. However, it can be useful when working with complex antigens because the precipitated antigen is normally detected after sodium dodecyl sulfate (SDS)-polyacrylamide electrophoresis and thus it is simple to discriminate between true and false positives. Furthermore, the assay provides information regarding the molecular weight of the antigen.
Keywords:
antigen capture;
hybridoma screening;
capture immunoprecipitation;
screening antigen;
immunoprecipitation
Journal Title: Cold Spring Harbor protocols
Year Published: 2022
Link to full text (if available)
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Journal Title: Cold Spring Harbor protocols
Year Published: 2022
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!