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By definition, a monoclonal antibody should only be of a single class or subclass. Each class of antibody is associated with specific functions, and it can be useful to know… Click to show full abstract
By definition, a monoclonal antibody should only be of a single class or subclass. Each class of antibody is associated with specific functions, and it can be useful to know the class/subclass of the monoclonal antibody produced by a specific hybridoma. In this protocol, class/subclass-specific antibodies are used to capture the monoclonal antibody from hybridoma supernatant. If the antibody is clonal, it will only be bound by one anti-heavy-chain capture antibody and one anti-light-chain antibody. No antigen is required.
Keywords:
class subclass;
class;
subclass monoclonal;
capture;
antibody
Journal Title: Cold Spring Harbor protocols
Year Published: 2022
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Journal Title: Cold Spring Harbor protocols
Year Published: 2022
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!