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Identifying the molecular mechanisms underlying antibiotic resistance is important, as it can reveal key information on the mode of action of a drug and provide insights for the development of… Click to show full abstract
Identifying the molecular mechanisms underlying antibiotic resistance is important, as it can reveal key information on the mode of action of a drug and provide insights for the development of novel or improved antimicrobials. Here, we describe an agar-based method for the selection of bacterial strains with increased antibiotic resistance, and how the increase in resistance can be confirmed by a spot-plating assay. As a specific example, we describe the selection of Staphylococcus aureus strains with increased resistance to oxacillin; however, the protocol can be easily adapted and used with other bacteria and antibiotics.
Journal Title: Cold Spring Harbor protocols
Year Published: 2023
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