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RNA Interference and Small RNA Analysis.

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Small silencing RNAs have provided powerful reverse genetics tools and have opened new areas of research. This introduction describes the use of RNAi to suppress expression of individual genes for… Click to show full abstract

Small silencing RNAs have provided powerful reverse genetics tools and have opened new areas of research. This introduction describes the use of RNAi to suppress expression of individual genes for loss-of-function analysis. It also summarizes methods for measuring specific and global changes in small RNA expression, as well as methods to inhibit the function of individual endogenous small RNA species such as miRNAs.

Keywords: small rna; interference small; rna analysis; rna; rna interference

Journal Title: Cold Spring Harbor protocols
Year Published: 2019

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