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Transcriptomics and Proteomics Methods for Xenopus Embryos and Tissues.

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The general field of quantitative biology has advanced significantly on the back of recent improvements in both sequencing technology and proteomics methods. The development of high-throughput, short-read sequencing has revolutionized… Click to show full abstract

The general field of quantitative biology has advanced significantly on the back of recent improvements in both sequencing technology and proteomics methods. The development of high-throughput, short-read sequencing has revolutionized RNA-based expression studies, while improvements in proteomics methods have enabled quantitative studies to attain better resolution. Here we introduce methods to undertake global analyses of gene expression through RNA and protein quantification in Xenopus embryos and tissues.

Keywords: transcriptomics proteomics; xenopus embryos; proteomics methods; methods xenopus; biology; embryos tissues

Journal Title: Cold Spring Harbor protocols
Year Published: 2019

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