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Experimental phase determination with selenomethionine or mercury-derivatization in serial femtosecond crystallography

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High-energy X-rays are essential for de novo structure determination with strong anomalous scattering from selenium or mercury. Single-wavelength anomalous diffraction phasing using selenomethionine-derivatization and mercury-soaking techniques has been successfully applied… Click to show full abstract

High-energy X-rays are essential for de novo structure determination with strong anomalous scattering from selenium or mercury. Single-wavelength anomalous diffraction phasing using selenomethionine-derivatization and mercury-soaking techniques has been successfully applied to serial femtosecond crystallography with 13.0 keV or 12.6 keV X-rays produced at SACLA.

Keywords: femtosecond crystallography; determination; serial femtosecond; derivatization; mercury

Journal Title: IUCrJ
Year Published: 2017

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