LAUSR

In order to real-time online monitoring the reaction substrates for biological processes, we designed an on-line Fourier transform infrared spectrometer measurement system, which has the advantages of low cost, simple and compact structure, good reliability, and strong shock resistance compared with the traditional Fourier transform infrared spectrometer. This system consists of two core components, i.e., a high-performance interferometer and an attenuated total reflectance (ATR) probe. In the interferometer, we 1) combined corner cube mirrors and flat mirrors to avoid tilting of the moving mirror; 2) set 60° incident angle to the ZnSe beam splitter to improve the luminous flux; 3) shared folded optical path with both the infrared light and the reference laser to improve compactness in structure; and 4) drive the moving mirror by a parallelogram swing flexible support module to make it move smoothly. In the ATR probe, we utilized two large diameter and inner coating light pipes to transmit the incident and outgoing light to enhance the luminous flux further. We also simulated the optical system and did some off-line and online measurement experiments. The results show the spectrometer has satisfactory reliability, large luminous flux, and online measurement capability. It can be real-time online monitoring the biological process through measuring the concentration of the related bioreactor reactant. The online analysis has wide application prospects in biological, chemical testing, material analysis, and many other fields.