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High Speed Three Dimensional Tracking of Swimming Cell by Synchronous Modulation Between TeCE Camera and TAG Lens

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This letter reports a prototype high-speed volume feedback microscope system, named a 1 ms 3D feedback microscope. In the proposed microscope system, three-dimensional information of the measured object can be acquired… Click to show full abstract

This letter reports a prototype high-speed volume feedback microscope system, named a 1 ms 3D feedback microscope. In the proposed microscope system, three-dimensional information of the measured object can be acquired by synchronizing the light emission from an LED and the movement of a fast variable-focus lens. An image corresponding to a particular focal length can be measured by flashing the LED when the lens is set at a particular focal length. When LEDs of multiple colors are modulated, a color image including multiple focal planes can be obtained. We constructed a prototype of the proposed microscope system and conducted experiments to confirm its effectiveness. Experimental results showed that the proposed microscope system could quickly measure three-dimensional information of the measured object.

Keywords: microscope system; microscope; high speed; three dimensional

Journal Title: IEEE Robotics and Automation Letters
Year Published: 2020

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