LAUSR

High-frequency ultrasound (HFUS) imaging is extensively used for cardiac diseases in small animals due to its high spatial resolution. However, there is a lack of a system that can provide a two-dimensional high-spatiotemporal dynamic visualization of mouse myocardial strains. In this paper, a dynamic HFUS (40 MHz) high-resolution strain imaging was developed through the vector Doppler imaging. Following in-vitro tests using a rubber balloon phantom, in vivo experiments were performed on wild-type (WT) and myocardial infarction (MI) mice. High-resolution dynamic images of myocardial strains were obtained in the longitudinal, radial, and circumferential directions at a frame rate of 1 kHz. Global peak strain values for WT mice were -19.3 ± 1.3 % (longitudinal), 31.4 ± 1.7 % (radial in the long axis), -19.9 ± 0.8 % (circumferential), and 34.4 ± 1.9 % (radial in the short axis); those for the MI mice were -16.1 ± 0.9 % (longitudinal), 26.8 ± 2.9 % (radial in the long axis), -15.2 ± 2.7 % (circumferential), and 21.6 ± 4.8 % (radial in the short axis). These results indicate that the strains for MI mice are significantly lower than those of WT mice. Regional longitudinal strain curves in the epicardial, midcardial and endocardial layers were measured and the peak strain values for WT mice were -22.0 % and -16.8 % in the endocardial and epicardial layers, respectively. However, no significant difference in the layer-based values was noted for the MI mice. Regional analysis results revealed obvious myocardial strain variation in the apical anterior region in the MI mice. The experimental results demonstrate that the proposed dynamic cardiac strain imaging can be useful in high performance imaging of small-animal cardiac diseases.