LAUSR

Objective To investigate if modification of a gonadotrophin‐releasing hormone (GnRH) based protocol to synchronise oestrus in Bos indicus cattle will improve response rates to the first administration of GnRH and improve pregnancy rates to artificial insemination (AI). Design Randomised control study. Methods Bos indicus heifers, nonlactating and lactating cows allocated to a GnRH‐18 treatment (n = 237) were treated with an intravaginal device (IVD) and cloprostenol (0.5 mg IM) on day −11 and on day 0 remaining animals in the GnRH‐7 treatment (n = 222) were administered an IVD and every animal was treated with GnRH (100 μg IM). On day 7, equine chorionic gonadotrophin (400 IU IM) and cloprostenol were administered and IVD's were removed. Animals detected in oestrus on day 9 were artificially inseminated while those not detected in oestrus were administered GnRH (100 μg IM) at 1700 hours and inseminated on day 10. Bulls were inserted 2 weeks after completion of AI and remained until day 65. Results The GnRH‐18 protocol increased the diameter of the largest follicle in the ovary on day 0, increased the percentage of new CL's induced after day 0 (46.3% vs 36.1%, for GnRH‐18 and GnRH‐7; P = 0.022), decreased circulating concentrations of progesterone on day 7 and increased odds of pregnancy to AI in heifers but not in nonlactating and lactating cows. Conclusion Treatment with the GnRH‐18 compared to the GnRH‐7 protocol increased pregnancy rates to AI in heifers but not in nonlactating or lactating cows.