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Novel strategy for primary epithelial cell isolation: Combination of hyaluronidase and collagenase I

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Different strategies for epithelial cell isolation significantly affect the viability and physiological properties of primary cells. Trypsin digestion, a conventional method, causes collateral damage owing to its strong digestive potential.… Click to show full abstract

Different strategies for epithelial cell isolation significantly affect the viability and physiological properties of primary cells. Trypsin digestion, a conventional method, causes collateral damage owing to its strong digestive potential. To better preserve the physiological properties of epithelial tissues, we aimed to develop a modified method (hyaluronidase and collagenase I combination) for primary cell isolation.

Keywords: cell isolation; cell; hyaluronidase collagenase; epithelial cell

Journal Title: Cell Proliferation
Year Published: 2022

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