LAUSR

Cytological specimens may be the ideal source of nucleic acids for the molecular characterization of tumors in the next generation sequencing (NGS) era. Previous experiences demonstrated good performances in terms of DNA extraction from disparate cytological samples, even proving their superiority on formalin fixed paraffin embedded (FFPE) material. Here is reported a further possible source for NGS studies, represented by the fluids taken by gentle mechanical disaggregation in saline solution of fresh core needle biopsies (liquid-based shaking). This method could lead to obtain both viable cells suitable for flow cytometry and fluorescence in situ hybridization (FISH) as well as free subcellular material (e.g. DNA) for molecular analysis (e.g. Sanger sequencing/real time PCR/ NGS), helping to overcome the issue of scant FFPE material after several procedures for the traditional characterization of neoplasms (i.e., immunohistochemistry).