LAUSR

BACKGROUND Leaky gut syndrome (LGS) is an idiopathic disorder characterised by alterations in intestinal permeability and low-grade systemic inflammation. Factors contributing to development of LGS are not well-understood but physiological stressors such as exercise and transport may play a role which may be of pathophysiological relevance in horses. OBJECTIVES To characterise the combined effect of transport stress and exercise on gastrointestinal permeability, and to determine whether these effects are associated with increased inflammatory biomarkers in plasma. STUDY DESIGN Controlled, randomised, cross-over study. METHODS Horses (n=8 per group) were given a gastrointestinal permeability tracer (iohexol; 5.6% solution; 1mL/kg bwt) via nasogastric entubation prior to being assigned to a stressed (EX; one hour of trailer transport immediately followed by 30 minutes moderate intensity exercise; n=4) or sedentary control (CON; n=4) group. Plasma samples were obtained prior to iohexol administration (P1), after transport (P2), at exercise cessation (P3), and at 1 (P4), 2 (P5), 4 (P6) and 8 (P7) hours after cessation of exercise and were analysed for iohexol, inflammatory biomarkers (SAA, LPS, IFABP, LBP) and tight junction proteins (zonulin). Fecal samples were collected at times corresponding to before and after stress from both groups and analysed for zonulin. Data were analysed using a 2-way RM ANOVA. RESULTS In EX horses, a significant increase in iohexol was observed at P2 (1.5±0.24μg/ml; p=0.03), P3 (2.1±0.29μg/ml; p<0.001), P4 (2.1±0.17μg/ml; p<0.001) compared with P1 (0.7±0.21μg/ml); iohexol was significantly higher in EX than CON horses at P3 (p<0.001), P4 (p<0.001) and P5 (p=0.003). LPS and SAA were significantly higher in EX than CON at P4 (p<0.001) and P6 (p=0.04), respectively. MAIN LIMITATIONS Data from our small sample size may not be generalisable to the larger equine population. CONCLUSIONS Combined transport and exercise increases gastrointestinal permeability and systemic SAA and LPS. The model described herein may be useful in further studies on the role of alterations in gastrointestinal permeability in equine disease. This article is protected by copyright. All rights reserved.