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Media pH and media type can significantly affect the reliability of in vitro copper tolerance assessments of Pseudomonas syringae pv. tomato

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There are inconsistencies with in vitro copper tolerance screening methodology for Pseudomonas syringae pv. tomato in the current literature, particularly in relation to the appropriate medium to use, copper tolerance… Click to show full abstract

There are inconsistencies with in vitro copper tolerance screening methodology for Pseudomonas syringae pv. tomato in the current literature, particularly in relation to the appropriate medium to use, copper tolerance thresholds and reporting medium pH and/or pH adjustment steps. This study investigates the effect of medium and pH on copper tolerance results, including the potential use of 2‐(N‐morpholino)ethanesulfonic acid (MES) buffer to stabilize medium pH.

Keywords: syringae tomato; copper tolerance; copper; vitro copper; pseudomonas syringae

Journal Title: Journal of Applied Microbiology
Year Published: 2018

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