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The detection and enumeration of Legionella spp. in water samples are typically performed via a cultural technique standardized in ISO 11731. This method is time‐consuming (up to 15 days), and… Click to show full abstract
The detection and enumeration of Legionella spp. in water samples are typically performed via a cultural technique standardized in ISO 11731. This method is time‐consuming (up to 15 days), and the specificity of the confirmation step is questionable. This study proposes the use of multiplex polymerase chain reaction (PCR) to confirm presumptive Legionella colonies directly from the culture plate; this shortens the response time by 2–5 days while still reporting results in colony forming units (CFU).
Keywords:
method;
pcr;
legionella;
legionella spp;
comparison culture
Journal Title: Journal of Applied Microbiology
Year Published: 2021
Link to full text (if available)
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Journal Title: Journal of Applied Microbiology
Year Published: 2021
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!