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Dead or alive: microbial viability treatment reveals both active and inactive bacterial constituents in the fish gut microbiota

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This study evaluated the microbial viability of fish gut microbiota in both digesta (faecal) and mucosal samples using a modified propidium monoazide (PMA) protocol, followed by 16S ribosomal RNA (rRNA)… Click to show full abstract

This study evaluated the microbial viability of fish gut microbiota in both digesta (faecal) and mucosal samples using a modified propidium monoazide (PMA) protocol, followed by 16S ribosomal RNA (rRNA) gene sequencing.

Keywords: fish gut; microbial viability; gut microbiota; dead alive

Journal Title: Journal of Applied Microbiology
Year Published: 2021

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