LAUSR

AIMS To investigate anti-inflammatory effects of Lactobacillus reuteri LM1071 in lipopolysaccharides (LPS)-induced inflammation RAW264.7 cells. METHODS AND RESULTS To evaluate anti-inflammatory activities of L. reuteri LM1071, LPS-stimulated RAW264.7 cells were used. Gene expression levels of eight immune-associated genes including IL-1β, IL-6, and TNF-α and protein production levels of COX-1 and COX-2 were analyzed. Moreover, the production of eicosanoids as important biomarkers for anti-inflammation was determined. CONCLUSIONS The current study demonstrates that L. reuteri LM1071 has anti-inflammatory potential by inhibiting the production of inflammation mediators such as NO, eicosanoids such as PGE1 & PGE2, pro-inflammatory cytokines, and COX proteins. It can also enhance the production of inflammatory associated genes such as IL-11, BMP4, LEFTY2, and EET metabolite. SIGNIFICANCE AND IMPACT OF THE STUDY Lactobacillus reuteri is one of crucial bacteria for food fermentation. It can be found in the gastrointestinal system of human and animals. Several studies have shown that L. reuteri has valuable effects on host health. The current study firstly demonstrated that L. reuteri has beneficial effect on the inflammation containing the variation of eicosanoids which are the one of the most important biomarkers and moreover eicosanoid-associated genes as well as proteins.