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Discrimination of Euglena gracilis strains Z and bacillaris by MALDI‐TOF MS

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Euglena gracilis is used as model organism for various microbiological, molecular biological and biotechnological studies. Its most studied wild‐type strains are Z and bacillaris, but their discrimination by standard molecular… Click to show full abstract

Euglena gracilis is used as model organism for various microbiological, molecular biological and biotechnological studies. Its most studied wild‐type strains are Z and bacillaris, but their discrimination by standard molecular methods is difficult. Therefore, we decided to test the suitability of MALDI‐TOF MS (matrix‐assisted laser desorption/ionization–time of flight mass spectrometry) for identification of E. gracilis and for discrimination of these two strains possessing functional chloroplasts. MALDI‐TOF MS profiling was also tested for two white (non‐photosynthetic) stable E. gracilis mutant strains WgmZOflL and W10BSmL.

Keywords: tof; euglena gracilis; maldi tof; discrimination; strains bacillaris

Journal Title: Journal of Applied Microbiology
Year Published: 2022

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