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Flexible multiplex PCR to detect SARS‐CoV‐2, coronavirus OC43 and influenza A virus in nasopharyngeal swab samples

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Quantitative reverse transcription PCR (RT‐qPCR) is the leading tool to detect severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2). Given that it will almost certainly continue to coexist with other respiratory… Click to show full abstract

Quantitative reverse transcription PCR (RT‐qPCR) is the leading tool to detect severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2). Given that it will almost certainly continue to coexist with other respiratory viruses in the coming years, our study aimed to design a multiplex PCR system not affected by supplier outages and with reduced cost compared to the existing commercially available kits.

Keywords: coronavirus; pcr; detect; flexible multiplex; sars cov; multiplex pcr

Journal Title: Journal of Applied Microbiology
Year Published: 2022

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