LAUSR

AIM The aim of this study was to evaluate the effect of the administration of pasteurized A. muciniphila and Amuc_1100 on periodontal destruction in lean and obese mice and to determine the impact of the mode of administration. MATERIALS AND METHODS Porphyromonas gingivalis associated experimental periodontitis was induced in lean and obese mice. After three weeks, the administration of live, pasteurized A. muciniphila or Amuc_1100 was performed by oral or gastric gavage for three additional weeks. Moreover, an evaluation of the interaction between A. muciniphila and P. gingivalis was performed by RNA-sequencing and cytokines secretion was measured in exposed macrophages. RESULTS Oral administration of live, pasteurized A. muciniphila or Amuc_1100 significantly decreased P. gingivalis-induced periodontal destruction and inflammatory infiltrate in lean and obese and contributed to reduce plasmatic level of TNF-α and increase IL-10. The co-culture of A. muciniphila and P. gingivalis induced an increased expression of genes linked to the synthesis of monobactam related antibiotics in A. muciniphila while a decrease of the gingipains and type IX secretion system was observed in P. gingivalis. In P. gingivalis infected macrophages, pasteurized A. muciniphila decreased TNF-α and increased IL-10 levels. CONCLUSION Pasteurized A. muciniphila can counteract P. gingivalis associated periodontal destruction. This article is protected by copyright. All rights reserved.