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Several research groups have reported methods for quantifying pancreatic beta cell (β‐cell) injury by measuring β‐cell‐specific CpG unmethylation of the insulin gene in circulation using digital droplet PCR or next‐generation… Click to show full abstract
Several research groups have reported methods for quantifying pancreatic beta cell (β‐cell) injury by measuring β‐cell‐specific CpG unmethylation of the insulin gene in circulation using digital droplet PCR or next‐generation sequencing. However, these methods have certain disadvantages, such as the need to consider the background signal owing to the small number of target CpG sites and the need for unique equipment.
Keywords:
cell;
conversion dual;
utilizing bisulfite;
bisulfite conversion;
novel method;
method utilizing
Journal Title: Journal of Diabetes Investigation
Year Published: 2022
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Journal Title: Journal of Diabetes Investigation
Year Published: 2022
Link to full text (if available)
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recommendations!