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Effects of anticoagulants on stable-isotope values (δ13 C and δ15 N) of shark blood components.

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The effects of anticoagulant EDTA and sodium heparin (SH) on stable carbon δ13 C and nitrogen δ15 N isotopic values of red blood cells (RBC) and blood plasma in juvenile… Click to show full abstract

The effects of anticoagulant EDTA and sodium heparin (SH) on stable carbon δ13 C and nitrogen δ15 N isotopic values of red blood cells (RBC) and blood plasma in juvenile blacktip reef sharks Carcharhinus melanopterus were analysed. Plasma preserved with anticoagulants was not isotopically distinct from plasma stored in no-additive control tubes but RBC δ15 N values exhibited small enrichments when preserved with EDTA and SH. Results suggest EDTA and SH are viable anticoagulants for stable isotopic analyses of blood fractions but further studies are advised to validate results. This article is protected by copyright. All rights reserved.

Keywords: anticoagulants stable; values shark; blood; stable isotope; isotope values; effects anticoagulants

Journal Title: Journal of fish biology
Year Published: 2019

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