LAUSR

Curcuminoids and tea polyphenols (CTP) are nutraceuticals with anticancer activity. To evaluate their combined in vitro anti-cancer activity, the CTP mixture was loaded on solid lipid nanoparticles (SLNPs). The resulting CTP-SLNPs were characterized and shown to have good chemical stability, making them suitable for delivery to cancer cells. A validated HPLC-MS method was developed to monitor the composition and quality of the prepared CTP-SLNP formulation, by identifying and quantifying its eight main components (epigallocatechin, epicatechin, catechin, epigallocatechingallate, epicatechingallate, curcumin, demethoxycurcumin and bisdemethoxycurcumin). The in vitro activity of CTP-SLNPs was evaluated and shown to significantly inhibit the proliferation of HepG2 and CT-26 cancer cells lines, indicating that anti-cancer properties of curcuminoids and tea polyphenols were enhanced in the CTP-SLNPs formulation. The results of this work are promising for further investigation and development of CTP-SLNPs as nanomedicines for cancer. Practical applications This research presents a practical approach for utilizing the anti-cancer benefits of natural bioactive ingredients from tea and turmeric, and has potential applications in clinical cancer therapy. The present work provides a stable CTP-SLNPs formulation with significant anti-cancer activity, which could be used for further development as a potential chemotherapeutic agent. The validated analytical method presented here can be used for characterization and comprehensive quality control of the novel CTP-SLNPs formulation.