LAUSR

In the current study, some physicochemical, mechanical, thermal, structural, and antibacterial properties of hydroxypropyl methyl cellulose–whey protein concentrate (HPMC–WPC) film loaded with chitosan nanoparticles (CNPs) are scrutinized. Initially, the optimal ratio of HPMC:WPC (70:30) was screened, then CNPs loaded into the HPMC:WPC blend film at the levels of 1%–5%. Well compatibility of the blend film components and generation intermolecular interactions were demonstrated by Fourier transform infrared and X‐ray diffraction tests, respectively. A uniform distribution of CNPs within the film structure was monitored by scanning electron microscopy micrographs. The DSC scans showed an upgrading in thermal characterizations of the blend film as a result of CNPs addition. As well, the water vapor permeability and flexibility of bionanocomposites decreased, while the tensile strength improved considerably (p < .05). The antibacterial effects of CNPs was more evident on Gram‐positive (Listeria monocytogenes and Staphylococcus aureus) rather than Gram‐negative (Escherichia coli) bacteria. PRACTICAL APPLICATIONS: The current research would have various uses in the area of food packaging. Bio‐based packaging films are developed to substitute petrol‐based materials for health and ecological reasons. Nanoparticle loading and blending of plant‐based biopolymer films are strategies can strength their physical, mechanical, structural, and antibacterial attributes.