LAUSR

BACKGROUND Candida albicans is linked to persistent endodontic lesions. However, the recognition receptor that identifies it is not explored previously. OBJECTIVES The aim of this study was to (1) establish a zymosan induced model of apical periodontitis in mouse, (2) observe the expression of Dectin-1 and its possible relationship with toll like receptor (TLR) 2 and (3) observe relationship between Osteopontin (OPN) and inflammatory cytokines. METHODS A total of 138 Naval Medical Research Institute (NMRI) mice were randomly divided into; Experimental Group n=69 and Zymosan Group n=69. Periapical periodontitis was developed in right maxillary molar. The animals were sacrificed at 7, 21 and 42 days. Bone blocks containing the mesial root (n=15 for qRT-PCR, n=45 for enzyme linked immune sorbent assay (ELISA)) were collected for mRNA expression and ELISA. While whole maxilla (n=3 from each time interval) were used for histology and Immunohistochemical analysis. One way Analysis Of Variance (ANOVA) and Tuckey's posthoc was used for statistical analysis at p ≤0.05. RESULTS TLR-2, Dectin-1 and TLR4-positive cells was detected at all time intervals in both groups. A strong postive correlation was observed between TLR-2 and Dectin-1 in both lesions (Regular r=0.680, p=0.015, Zymosan (r=0.861, p<0.001)). A significant correlation was found between OPN and Tumor Necrosis Factor-alpha (TNF-α) in Zymosan lesion (r= 0.827, p=0.001). CONCLUSIONS Immune cells of inflamed periapical tissue expressed Dectin-1 receptor in response to the microbial challenge from infected root canals and showed positive correlation with TLR-2 and OPN suggesting a possible receptor collaboration mediated by OPN. The expression of OPN and TNF-α showed positive correlation in response to fungal antigen, indicating a possible relationship.