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PURPOSE To evaluate the effect of the application sequence of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) paste (MI paste) on shear bond strength (SBS) of 2 etch-and-rinse resin cements to dentin. MATERIALS AND METHODS Sixty extracted human maxillary premolars were cut to expose the mid-coronal dentin surfaces. The teeth were randomly divided into 6 groups (n = 10), based on the resin cement used and CPP-ACP application. In groups 1-3, Excite DSC/Variolink II and in groups 2-4, One-Step Plus/Duolink was bonded to dentin. There were 3 groups for each cement as follows: with no treatment as a control; 3-minute application of CPP-ACP before acid etching (CPP-ACP/Etching); 3-minute application of CPP-ACP after acid etching (Etching/CPP-ACP). After storage in distilled water at 37°C for 24 hours, SBS test was performed. Data were analyzed with 2-way ANOVA and post-hoc Tukey test (α = 0.05). RESULTS CPP-ACP application on the dentin before and after acid etching significantly decreased the SBS of OS/Duolink (13.60 and 14.71 MPa, respectively vs. control; 21.21 MPa, p < 0.001); however, it did not influence the SBS of Excite DSC/Variolink II (25.14 MPa) before acid etching (27.37 MPa) or after acid etching (26.41 MPa, p > 0.388). The application sequence of CPP-ACP did not influence the SBS of etch-and-rinse resin cements evaluated. CONCLUSION CPP-ACP applications may lower the SBS in some etch-and-rinse resin cements, and timing of applications makes no difference.