LAUSR

In this study, we aimed to analyse the spoilage potential of the isolated yeast, LAB and AAB species. Thus, eleven strains were inoculated at 0.3% (v/v) into a sterile filtered tchapalo and stored for three days at ambient temperature (27-30°C). All the tested strains grew well or remained stable except for Limosilactobacillus fermentum and Pediococcus acidilactici which decreased throughout the storage time. A significant decrease of Total Soluble Solids was observed only for Saccharomyces cerevisiae (from 7.8 to 5.8 °Brix) and M. guilliermondii (from 7.8 to 5.5 °Brix). The tchapalo samples inoculated with the LAB strains Weissella paramesenteroides, P. acidilactici, Limosilactobacillus fermentum and the yeast strain Candida tropicalis were judged similar to the control by the panellists. However, the strains of Lacticaseibacillus paracasei and Latilactobacillus curvatus (LAB), S. cerevisiae, Meyerozyma guilliermondii and Kluyveromyces marxianus (yeasts) and Acetobacter pasteurianus and A. cerevisiae (AAB) induced the spoilage of the tchapalo appearance, smell and/or taste. In the spoiled tchapalo quantitative and qualitative modification of some volatile compounds (VOCs) such as lilac aldehyde, ethyl acetate, ethyl hexanoate, ethyl octanoate and phenethyl acetate, were observed. These results provide information about the microorganisms that need to be removed to extend the shelf life of tchapalo.