LAUSR

The COVID‐19 pandemic has raised interest in using devices that generate ultraviolet C (UVC) radiation as an alternative approach for reducing or eliminating microorganisms on surfaces. Studies investigating the efficacy of UVC radiation against pathogens use a wide range of laboratory methods and experimental conditions that can make cross‐comparison of results and extrapolation of findings to real‐world settings difficult. Here, we use three different UVC‐generating sources – a broad‐spectrum pulsed xenon light, a continuous light‐emitting diode (LED), and a low‐pressure mercury vapour lamp – to evaluate the impact of different experimental conditions on UVC efficacy against the coliphage MS2 on surfaces. We find that a nonlinear dose–response relationship exists for all three light sources, meaning that linear extrapolation of doses resulting in a 1‐log10 (90%) reduction does not accurately predict the dose required for higher (e.g. 3‐log10 or 99.9%) log10 reductions. In addition, our results show that the inoculum characteristics and underlying substrate play an important role in determining UVC efficacy. Variations in microscopic surface topography may shield MS2 from UVC radiation to different degrees, which impacts UVC device efficacy. These findings are important to consider in comparing results from different UVC studies and in estimating device performance in field conditions.