LAUSR

Soil microbiota increase their fitness to local habitats by adjusting their life history strategies. Yet, how such adjustments drive their ecological adaptations in xeric grasslands remains elusive. In this study, shifts in the traits that potentially represent microbial life history strategies were studied along two aridity gradients with different climates using metagenomic and trait-based approaches. The results indicated that resource acquisition (e.g., higher activities of β-D-glucosidase and N-acetyl-beta-D-glucosidase, higher degradation rates of cellulose and chitin as well as genes involved in cell motilities, biodegradations, transportations and competitions) and growth yield (e.g., higher biomass and respiration) strategies were depleted with a higher aridity. However, cellular and high growth potential maintenance (e.g., higher metabolic quotients and genes related to DNA replication, transcription, translation, central carbon metabolisms and biosynthesis) and stress tolerance (e.g., genes involved in DNA damage repair, cation transporter, sporulation and osmolyte biosynthesis) strategies were enriched with a higher aridity. This implied that microbiota have lower growth yields but are probably well primed for rapid responses to pulse rainfalls in more arid soils, whereas those in less arid soils may have stronger resource acquisition and growth yield abilities. By integrating a large amount of evidence involved in taxonomic, metagenomic, genomic and biochemical properties, this study demonstrated that the ecological adaptations of soil microbiota to aridity made by adjusting and optimizing their life history strategies are universal in xeric grasslands and provided an underlying mechanistic understanding of soil microbial responses to climate changes.