LAUSR

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission can occur with saliva and aerosol droplets deriving from the upper aerodigestive tract during coughing, sneezing, talking, and even during oral inspection or dental procedures. The aim of this study was to assess in vitro virucidal activity of commercial and experimental mouthwashes against a feline coronavirus (FCoV) strain. Commercial and experimental (commercial-based products with addition of either sodium dodecyl sulfate (SDS) or thymus vulgaris essential oil (TEO) at different concentrations) mouthwashes were placed in contact with FCoV for different time intervals i.e., 30 seconds (T30), 60 seconds (T60) and 180 seconds (T180); subsequently the virus was titrated on Crandell Reese Feline kidney cells. An SDS-based commercial mouthwash reduced the viral load by 5 log10 Tissue Culture Infectious Dose (TCID)50 /50µl at T30 whilst a cetylpyridinium (CPC)-based commercial mouthwash was able to reduce the viral titer of 4.75 log10 at T60. Furthermore, 5 experimental mouthwashes supplemented with SDS reduced the viral titer by 4.75-5 log10 according to a dose (up to 4mM) and time-dependent fashion.