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The moss Physcomitrella patens is a valuable system to address evolutionary changes of plants (Prigge and Bezanilla, 2010). Till date, functional studies in P. patens have been mainly carried out… Click to show full abstract
The moss Physcomitrella patens is a valuable system to address evolutionary changes of plants (Prigge and Bezanilla, 2010). Till date, functional studies in P. patens have been mainly carried out by gene knockout owing to its high rate of homologous recombination (HR) (Schaefer and Zrÿd, 1997). However, replacement of redundant genes sequentially by HR is laborious and time-consuming. This article is protected by copyright. All rights reserved.
Keywords:
cas9 oligonucleotide;
transient cotransformation;
cotransformation crispr;
physcomitrella;
physcomitrella patens;
crispr cas9
Journal Title: Plant Biotechnology Journal
Year Published: 2019
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Journal Title: Plant Biotechnology Journal
Year Published: 2019
Link to full text (if available)
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recommendations!