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Targeted Generation of Null Mutants in ZmGDIα Confers Resistance against Maize Rough Dwarf Disease without Agronomic Penalty.

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In summary, our data indicate that both null mutants generated through CRISPR/Cas9 editing exhibit stronger resistance against MRDD than the natural ZmGDIα-hel allele. Our study also alleviates any concerns about… Click to show full abstract

In summary, our data indicate that both null mutants generated through CRISPR/Cas9 editing exhibit stronger resistance against MRDD than the natural ZmGDIα-hel allele. Our study also alleviates any concerns about the possible agronomic penalty associated with engineering. Targeted editing of Rab GDIα might be extended in other monocot crops to engineer RBSDV resistance.

Keywords: agronomic penalty; null mutants; targeted generation; generation null; mutants zmgdi

Journal Title: Plant biotechnology journal
Year Published: 2022

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