LAUSR

genetic screens are excellent tool to assign gene function, but it is often necessary to employ map-based cloning to identify the causal genes. This can be laborious and represents a bottleneck in in DNA it is becoming increasingly afford-able to sequence large populations. Krasileva et al . (2017) exome sequenced tetraploid and hexaploid wheat ethyl methanesul-fonate (EMS) mutagenized populations, primarily to facilitate reverse genetic screens. Gene redundancy allows a very high mutant load of 35 – 40 mutations per kilobase, and the populations of ~ 1500 and ~ 1200 lines each harbour ~ 22 – 23 missense or truncation mutations per gene. Here, we show that burden tests, a simple form of rare-variant association analysis developed for human disease genetics (Lee et al ., 2014), can be used to identify causal genes in the hexaploid wheat ( Triticum aestivum ) cv. Cadenza mutant population, without the need for map-based cloning. power with is ., and in the Cadenza singletons .,